| Value |
9.6e+5
M^-1×sec^-1
Range: Table - link M^-1×sec^-1
|
| Organism |
Bacteriophage T7 |
| Reference |
McClure WR. Rate-limiting steps in RNA chain initiation. Proc Natl Acad Sci U S A. 1980 Oct77(10): 5634-8.PubMed ID6160577
|
| Method |
Promoter-specific lags in the approach to the steady-state rate of abortive initiation were observed when Escherichia coli RNA polymerase was added to initiate the reaction. The time required for a promoter and RNA polymerase to
combine and isomerize into an RPO (RNA polymerase-promoter open complex) under a particular set of
experimental conditions can be measured by initiating the
promoter-specific abortive initiation reaction with RNA
polymerase. The kinetic constants kon and k2 were evaluated from the slopes
and intercepts of Fig. 2 (Tau observed vs 1/[RNAP], Tau observed is the average time required
for open complex formation). |
| Comments |
The model of the reaction is R+P><RPc><RPo, where R is RNAP, P promoter, RPc RNAP-promoter closed complex, RPo RNAP-promoter open complex |
| Entered by |
Uri M |
| ID |
105134 |