| Range |
0.042-0.074 µm^3
|
| Organism |
Bacterioplankton |
| Reference |
Schut F, de Vries EJ, Gottschal JC, Robertson BR, Harder W, Prins RA, Button DK. Isolation of Typical Marine Bacteria by Dilution Culture: Growth, Maintenance, and Characteristics of Isolates under Laboratory Conditions. Appl Environ Microbiol. 1993 Jul59(7):2150-2160. Table - link PubMed ID16348992
|
| Method |
Flow cytometry was performed on a
Ortho Cytofluorograf IIS as described previously (71), except
that samples were preserved with 0.5% formaldehyde,
stored cold, made permeable by treatment with 0.1% Triton X-100, and stained with DAPI (0.5 ,ug/ml) at 10°C for 1 h
before analysis. Data were analyzed as described previously
(16) and are plotted in bivariate histograms of apparent DNA
per cell versus cell volume. |
| Comments |
Marine bacteria in Resurrection Bay near Seward, Alaska, and in the central North Sea off the Dutch coast
were cultured in filtered autoclaved seawater following dilution to extinction. The populations present before
dilution varied from 0.11x10^9 to 1.07x10^9 cells per liter. The mean cell volume varied between 0.042 and
0.074 µm^3, and the mean apparent DNA content of the cells ranged from 2.5 to 4.7 fg of DNA per cell. All three
parameters were determined by high-resolution flow cytometry. Since the extinction dilution method favors the growth of
predominant species, the cultures generated from small
inocula (<10 cells) can be addressed as cultures of typical
marine bacteria. The isolates obtained from these dilutions
are indeed similar in size and apparent DNA content to those
of the bacteria normally present in seawater |
| Entered by |
Uri M |
| ID |
105117 |