| Range |
0.11×10^9-1.07×10^9 cells/ml
|
| Organism |
Bacterioplankton |
| Reference |
Schut F, de Vries EJ, Gottschal JC, Robertson BR, Harder W, Prins RA, Button DK. Isolation of Typical Marine Bacteria by Dilution Culture: Growth, Maintenance, and Characteristics of Isolates under Laboratory Conditions. Appl Environ Microbiol. 1993 Jul59(7):2150-2160. Table - link PubMed ID16348992
|
| Method |
The population density and morphology of the cultures
were determined by flow cytometry and epifluorescence
microscopy. |
| Comments |
Marine bacteria in Resurrection Bay near Seward, Alaska, and in the central North Sea off the Dutch coast
were cultured in filtered autoclaved seawater following dilution to extinction. The populations present before
dilution varied from 0.11x10^9 to 1.07x10^9 cells per liter. The mean cell volume varied between 0.042 and
0.074 µm^3, and the mean apparent DNA content of the cells ranged from 2.5 to 4.7 fg of DNA per cell. All three
parameters were determined by high-resolution flow cytometry. Since the extinction dilution method favors the growth of
predominant species, the cultures generated from small
inocula (<10 cells) can be addressed as cultures of typical
marine bacteria. |
| Entered by |
Uri M |
| ID |
105119 |