| Value |
7.1E-09
substitution/site/generation
Range: ±0.7E-09 substitution/site/generation
|
| Organism |
Thale cress Arabidopsis thaliana |
| Reference |
Stephan Ossowski, Korbinian Schneeberger,
Jose Ignacio Lucas-Lled, Norman Warthmann,
Richard M. Clark, Ruth G. Shaw, Detlef Weigel and
Michael Lynch, The rate and molecular spectrum of spontaneous
mutations in Arabidopsis thaliana, Science. 2010 Jan 1 327(5961):92-94. p.92 right column 3rd paragraphPubMed ID20044577
|
| Method |
Researchers sequenced the genomes of five individuals derived by 30 generations of single-seed descent from the reference strain Col-0 (4), for which a high-quality genome was published in 2000 (5). They used the Illumina (Solexa) Genome Analyzer platform to obtain a depth of sequence coverage of between 23 and 31 in each MA line. Sequencing reads between 36 and 43 bp in length were aligned to the reference genome, from which over 1000 errors had been removed in a previous study (6). They identified single-base substitutions using two complementary methods: a ‘consensus’ approach, and the SNP caller function of SHORE (http://1001genomes.org/ downloads/shore.html) (7). |
| Comments |
Note: multiplying mutation rate (value in this entry) by genome size (BNID 104 000), [7×10^-9substitution/bp/generation]×[1.57×10^8bp]=1.099 mutations/genome/generation. From article: "If we assume that only nonsynonymous mutations and indels affecting coding regions are likely
to affect fitness, the diploid genomic rate of mutations affecting fitness would be 0.2±0.1 per
generation, which is not significantly different from previous estimates based on the evaluation
of fitness components in MA lines of A. thaliana (14, 15)." See diploid mutation rate of 0.28-0.42 deleterious mutations per genome per generation BNID 102439. For haploid mutation rate of 0.06 mutations per genome per generation see BNID 104473. |
| Entered by |
Uri M |
| ID |
105036 |