Homology length in gene-targeting cassette
| Value | 1.53 Kb |
|---|---|
| Organism | Corn Zea mays |
| Reference | Shukla VK, Doyon Y, Miller JC, DeKelver RC, Moehle EA, Worden SE, Mitchell JC, Arnold NL, Gopalan S, Meng X, Choi VM, Rock JM, Wu YY, Katibah GE, Zhifang G, McCaskill D, Simpson MA, Blakeslee B, Greenwalt SA, Butler HJ, Hinkley SJ, Zhang L, Rebar EJ, Gregory PD, Urnov FD. Nature. Precise genome modification in the crop species Zea mays using zinc-finger nucleases. Nature. 2009 May 21 459(7245):437-41.PubMed ID19404259 |
| Method | Because targeted genome modification in plants has been intractable, plant trait engineering remains a laborious, time-consuming and unpredictable undertaking. Researchers report a broadly applicable, versatile solution to this problem: the use of designed zinc-finger nucleases (ZFNs) that induce a double-stranded break at their target locus. They describe the use of ZFNs to modify endogenous loci in plants of the crop species Zea mays. |
| Comments | For average gene length of 4.2 kbp see BNID 105368 |
| Entered by | Aviva_Samach |
| ID | 105015 |