Kcat (turnover rate) of activation of isoleucine by human mitochondrial Leu tRNA Synthetase
| Value | 0.07 Sec^-1 Range: ±0.004 Table - link Sec^-1 |
|---|---|
| Organism | Human Homo sapiens |
| Reference | Lue SW, Kelley SO. An aminoacyl-tRNA synthetase with a defunct editing site. Biochemistry. 2005 Mar 1 44(8):3010-6PubMed ID15723544 |
| Method | Cloning and Preparation of tRNA Constructs, Preparation of hs mt, WT E. coli, and E. coli T252Y LeuRS, Isolation of hs mt tRNALeu and E. coli tRNALeu Misacylated with Isoleucine, Deacylation of hs mt Ile-tRNALeu and E. coli Ile-tRNALeu, Cross-Aminoacylation and Misaminoacylation Assays, |
| Comments | Kinetic parameters were determined at pH 7.5 and 37 °C. For value of 18 sec^-1 in E. coli LeuRS see BNID 104981 |
| Entered by | Uri M |
| ID | 104979 |