ATPase activities of F1 engineered for rotation
| Range | Table - link |
|---|---|
| Organism | Bacteria Escherichia coli |
| Reference | Nakanishi-Matsui M, Kashiwagi S, Hosokawa H, Cipriano DJ, Dunn SD, Wada Y, Futai M. Stochastic high-speed rotation of Escherichia coli ATP synthase F1 sector: the epsilon subunit-sensitive rotation. J Biol Chem. 2006 Feb 17 281(7):4126-31PubMed ID16352612 |
| Method | The ATPase activities of wild-type F1 and F1 engineered (2 nM) for rotation were assayed at 24 °C, and the rotation rates were estimated assuming three ATP molecules were hydrolyzed per rotation. The ATPase activity was also assayed in the presence of 100 nM epsilon subunit (epsilon ) or epsilon with cytochrome b562 connected at the carboxyl terminus (epsilon-cytochrome b562). |
| Entered by | Uri M |
| ID | 104898 |