Total phospolipids in GN80 strain

Value 89000 picomole/mg protein
Organism Bacteria Escherichia coli
Reference Ganong BR, Raetz CR. Massive accumulation of phosphatidic acid in conditionally lethal CDP-diglyceride synthetase mutants and cytidine auxotrophs of Escherichia coli. J Biol Chem. 1982 Jan 10 257(1):389-94 Table - link PubMed ID6273438
Method When 100 micrograms/ml erythromycin is added to nutrient agar plates, it is possible to obtain a 30-fold enrichment for cds mutants from a mutagen-treated stock, as judged by colony autoradiography. Using this approach researchers isolated 38 new cds mutants, nine of which are unable to grow at a culture pH greater than 8. Protein levels were calculated from the A550 of the culture
Comments At pH 6, the steady state level of the total liponucleotides was only 0.01596, with a deoxy/ribo ratio comparable to that of GN85 (Table link). When the pH was raised to 8.5, the phospholipid/protein ratio dropped gradually, and the liponucleotide level did not increase as in wild type cells furthermore, the ratio of dCDP-diglyceride to CDP-diglyceride did not change when GN80 was shifted to pH 8.5. The net result was a 7 -fold relative reduction of the total liponucleotide pool after 120 min at pH 8.5 (Table link). See BNID 104813
Entered by Uri M
ID 104814