| Value |
89000
picomole/mg protein
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Ganong BR, Raetz CR. Massive accumulation of phosphatidic acid in conditionally lethal CDP-diglyceride synthetase mutants and cytidine auxotrophs of Escherichia coli. J Biol Chem. 1982 Jan 10 257(1):389-94 Table - link PubMed ID6273438
|
| Method |
When 100 micrograms/ml erythromycin is added to nutrient agar plates, it is possible to obtain a 30-fold enrichment for cds mutants from a mutagen-treated stock, as judged by colony autoradiography. Using this approach researchers isolated 38 new cds mutants, nine of which are unable to grow at a culture pH greater than 8. Protein levels were calculated from the A550 of the culture |
| Comments |
At pH 6, the steady state level of the total
liponucleotides was only 0.01596, with a deoxy/ribo ratio comparable
to that of GN85 (Table link). When the pH was raised
to 8.5, the phospholipid/protein ratio dropped gradually, and
the liponucleotide level did not increase as in wild type cells
furthermore, the ratio of dCDP-diglyceride to CDP-diglyceride
did not change when GN80 was shifted to pH 8.5. The net
result was a 7 -fold relative reduction of the total liponucleotide
pool after 120 min at pH 8.5 (Table link). See BNID 104813 |
| Entered by |
Uri M |
| ID |
104814 |