| Comments |
P.3525 right column bottom paragraph: "Indeed, the cross-sectional nuclear areas measured in the small cell populations, and the large cell populations generated by CLN3 manipulation were significantly different from the nuclear areas measured in wild-type cells in the same medium (Figure 2A, Table 2, Student's t tests, p < 10^−15). The differences in the nuclear areas appeared to be roughly proportional to the differences in cell volume measured with a Coulter particle analyzer (Figure 2B). To estimate nuclear volumes, [investigators] assumed that the nuclei were spherical, which is true or nearly true (Winey et al., 1997). The N/C ratio determined from population averages was quite constant, such that nuclear volume was 6–8% of cell volume, even though these alterations in CLN3 activity generated twofold differences in average cell volume (Table 2)." See nuclear volume of 2.9 µm^3 BNID 100447, for fraction of nucleus out of total cell volume in HeLa see comments section of BNID 101402. For fraction of nucleus volume out of total erythrocyte volume in chicken see BNID 104642 |