| Range |
Table - link
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Bennett BD, Kimball EH, Gao M, Osterhout R, Van Dien SJ, Rabinowitz JD. Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli. Nat Chem Biol. 2009 Aug5(8):593-9 p.594 table 1PubMed ID19561621
|
| Method |
P.594 right column top paragraph: "[Researchers] quantified metabolites by Liquid chromatography-mass spectrometry (LC-MS/MS) using an isotope ratio-based approach (ref 8). As isotope-labeled standards for many metabolites are not available, they used uniformly 13C-labeled glucose medium to label the intracellular metabolome of E. coli (ref 25). This enabled the use of commercially available unlabeled compounds as internal standards." |
| Comments |
Abstract: "The total observed intracellular metabolite pool was approximately 300 mM (BNID 104678). A small number of metabolites dominate the metabolome on a molar basis, with glutamate being the most abundant." P.595 left column bottom paragraph: "The absolute cellular concentrations of 103 metabolites were determined in glucose-fed cultures, and most were also determined in glycerol- and acetate-fed cultures (for glucose-grown cells only, see Table 1 for complete data, including error estimates, see Supplementary Table 3)." P.596 left column bottom paragraph: "[Researchers] used the absolute concentration of metabolites in glucose-grown cultures (Table 1) to assess the extent of saturation of enzymes by their substrates, by comparing the measured concentrations to Km values taken from the BRENDA database (Fig. 2 and Supplementary Table 7)." |
| Entered by |
Uri M |
| ID |
104679 |