Table - link
||Bacteria Escherichia coli
||Bennett BD, Kimball EH, Gao M, Osterhout R, Van Dien SJ, Rabinowitz JD. Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli. Nat Chem Biol. 2009 Aug5(8):593-9 Supplementary information pp.7-10 Supplementary table 3PubMed ID19561621
||"[Investigators] quantified metabolites by LC-MS/MS [Liquid chromatography-mass spectrometry] using an isotope ratio-based approach (ref 8). As isotope-labeled standards for many metabolites are not available, [they] used uniformly 13C-labeled glucose medium to label the intracellular metabolome of E. coli (ref 25). This enabled the use of commercially
available unlabeled compounds as internal standards."
||"TMFA [Thermodynamic metabolic flux analysis] was performed largely as originally described [ref 14]. Measured metabolite concentrations (Supplementary Table 3) were used to constrain the TMFA model." The total observed intracellular metabolite pool was approximately 300 mM.