Time after hypoosmotic stress for nuclear translocation of AP-1 to occur

Value 30 min
Organism Human Homo sapiens
Reference Kim RD et al, Activator protein 1 activation following hypoosmotic stress in HepG2 cells is actin cytoskeleton dependent. J Surg Res. 2001 Oct100(2):176-82PubMed ID11592789
Method HepG2 cells were incubated for 1 h with or without 20 microM cytochalasin D, an actin disrupter, and were then exposed for up to 30 min to hypoosmotic medium (200 mOsm/L) to induce swelling. Tumor necrosis factor alpha (1.4 nM) and medium alone served as positive and negative controls, respectively. Nuclear proteins were isolated using a modified version of Dignam et al. [3, 23] . Electromobility shift assay (EMSA) measured nuclear AP-1. All experiments were performed in triplicate.
Comments Researchers hypothesized that hypoosmotic stress-induced activation of PKB and AP-1 in HepG2 cells is dependent on an intact actin cytoskeleton and subsequent FAK phosphorylation
Entered by Uri M
ID 104613