Lifetime of photon donor dye molecule Alexa Fluor 594
| Value | 5 nsec |
|---|---|
| Organism | Generic |
| Reference | Chung HS, Louis JM, Eaton WA. Feature Article: Experimental determination of upper bound for transition path times in protein folding from single-molecule photon-by-photon trajectories. Proc Natl Acad Sci U S A. 2009 Jul 21 106(29):11837-44 p.11839 left column top paragraphPubMed ID19584244 |
| Method | FRET trajectories were measured on single molecules of the dye-labeled, 56-residue 2-state protein GB1, immobilized on a glass surface via a biotin-streptavidin-biotin linkage. Characterization of individual emitted photons by their wavelength, polarization, and absolute and relative time of arrival after picosecond excitation allowed the determination of distributions of FRET efficiencies, donor and acceptor lifetimes, steady state polarizations, and waiting times in the folded and unfolded states. |
| Comments | Transition paths are a uniquely single-molecule property not yet observed for any molecular process in solution. The duration of transition paths is the tiny fraction of the time in an equilibrium single-molecule trajectory when the process actually happens. |
| Entered by | Uri M |
| ID | 104601 |