GFP folding time

Value 600 sec
Organism Generic
Reference Dietz H, Rief M. Exploring the energy landscape of GFP by single-molecule mechanical experiments. Proc Natl Acad Sci U S A. 2004 Nov 16 101(46):16192-7. p.16194 left column bottom paragraphPubMed ID15531635
Primary Source Zimmer M. Green fluorescent protein (GFP): applications, structure, and related photophysical behavior. Chem Rev. 2002 Mar102(3):759-81 & Tsien RY. The green fluorescent protein. Annu Rev Biochem. 1998 67: 509-44.PubMed ID11890756, 9759496
Comments "Moreover, comparing the folding time of GFP of 600 s (primary sources) with [researchers'] measured lifetime of 14 s suggests the state before mechanical unfolding has a positive free energy of at least 3.7kBT in contrast to ?G= -16 kBT, which is determined from bulk measurements (12)."
Entered by Uri M
ID 111269