Mechanical loads that GFP bear at pulling velocities of 300 nm/s before it unfolds

Value 104 pN Range: ±40 pN
Organism Generic
Reference Dietz H, Rief M. Exploring the energy landscape of GFP by single-molecule mechanical experiments. Proc Natl Acad Sci U S A. 2004 Nov 16 101(46):16192-7. p.16194 left column top paragraphPubMed ID15531635
Comments "The Ig domains of DdFLN [shortened actin crosslinker dictyostelium filamin] exhibit unfolding forces in a range between 40 and 100 pN (5), and GFP unfolding in this chimera construct should occur at higher extensions after the weakest Ig domains have already unfolded. In force extension traces obtained with DdFLN1-5–GFP (Fig. 1d), the GFP unfolding events are indeed shifted toward higher extensions. Nonetheless, both the length gain and the unfolding force are identical to Ig8–GFP. Results for the two constructs are therefore in agreement, and [researchers] conclude that at pulling velocities of 300nm/s GFP on average can bear mechanical loads of 104±40 pN (Fig. 1e) before it unfolds."
Entered by Uri M
ID 111270