Replication rate of core polymerase

Range 80-120 nucleotides/sec
Organism Bacteria Staphylococcus aureus
Reference Klemperer N, Zhang D, Skangalis M, O'Donnell M. Cross-utilization of the beta sliding clamp by replicative polymerases of evolutionary divergent organisms. J Biol Chem. 2000 Aug 25275(34):26136-43PubMed ID10851235
Method This study examines replicase components for their ability to be switched between Gram-positive and Gram-negative organisms.
Comments Core contains the DNA polymerase and proofreading exonuclease activities. Please note- the following ref gives replication rate of 20 nts/sec for core polymerase: Kelman Z, O'Donnell M. DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine. Annu Rev Biochem. 1995 64:171-200 PUBMED ID: 7574479.
Entered by Uri M
ID 104393