Half life of cation-independent mannose-6-phosphate receptor (CI-MPR) protein in HeLa cell
| Value | 26 hours |
|---|---|
| Organism | Human Homo sapiens |
| Reference | Ganley IG, Carroll K, Bittova L, Pfeffer S. Rab9 GTPase regulates late endosome size and requires effector interaction for its stability. Mol Biol Cell. 2004 Dec15(12):5420-30.PubMed ID15456905 |
| Method | Antibodies and Recombinant Proteins, Cell Culture of HeLa cells, Immunoblot Analysis, Immunofluorescence Microscopy, Antibody Internalization Assay, Whole-Mount Electron Microscopy, Immunoelectron Microscopy, Metabolic Labeling and Immunoprecipitation, Epidermal Growth Factor Receptor (EGFR) Down-Regulation, TIP47/Rab9 Binding Studies, Crude Membrane Fractionation. To explore the importance of Rab9 for microdomain establishment, researchers depleted the protein from cultured cells. Rab9 depletion decreased late endosome size and reduced the numbers of multilamellar and dense-tubule–containing late endosomes/lysosomes, but not multivesicular endosomes. |
| Comments | In biochemistry and cell biology, the insulin-like growth factor 2 receptor (IGF2R), also called the cation-independent mannose-6-phosphate receptor (CI-MPR), is a multifunctional protein receptor that binds insulin-like growth factor 2 (IGF2) at the cell surface and mannose-6-phosphate (M6P)-tagged proteins in the trans-Golgi network. See BNID 104381,104386 |
| Entered by | Uri M |
| ID | 104380 |