| Range |
2.1 - 6.0 Table - link min
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Bernstein JA, Lin PH, Cohen SN, Lin-Chao S. Global analysis of Escherichia coli RNA degradosome function using DNA microarrays. Proc Natl Acad Sci U S A. 2004 Mar 2 101(9):2758-63 p.2759 table 1PubMed ID14981237
|
| Method |
Abstract: "To investigate the degradosome's proposed role as an RNA decay machine, [researchers] used DNA microarrays to globally assess alterations in the steady-state abundance and decay of 4,289 E. coli mRNAs at single-gene resolution in bacteria carrying mutations in the degradosome constituents RNase E, polynucleotide phosphorylase, RhlB helicase, and enolase." P.2759 caption to table 1: "Generation times were determined for each mutant strain and its respective parental strain by following growth at 30°C in M9 media supplemented as described in Materials and Methods by following culture density at OD540. Strain K10 is the parental strain of DF261. N3433 is the parental strain of both SU02 and YHC012. SH3208 is the parental strain of BZ453. Median mRNA half-lives for each strain are expressed in minutes. Indicated 95% confidence intervals were estimated as described in Materials and Methods." |
| Comments |
P.2761 left column 2nd paragraph: "Comparison of median mRNA half-life between strains encoding Eno, RhlB, Pnp and Rne mutant proteins and their respective parental strains showed prolongation of the median half-life
for all four mutants (Table 1 and Fig. 2)." Table gives median mRNA half life and generation times of different strains. For average mRNA half life of 1min at 37°C see Moselio Schaechter, John L. Ingraham, Frederick C. Neidhardt, Microbe, 2006 ASM Press 1 edition p.147 top paragraph. See BNID 100205 |
| Entered by |
Uri M |
| ID |
104324 |