Plastocyanin concentration in log phase
Value | 1 μmole/mole chlorophyll |
---|---|
Organism | Green algae Chlamydomonas reinhardtii |
Reference | Wood PM. Interchangeable copper and iron proteins in algal photosynthesis. Studies on plastocyanin and cytochrome c-552 in Chlamydomonas. Eur J Biochem. 1978 Jun 187(1):9-19 Table - link PubMed ID208838 |
Method | For chlorophyll assays the material was added to acetone and hand homogenised. For complete extraction of soluble proteins (plastocyanin and cytochromes c-552 and c-550) the cells were spun down, washed in 10 mM phosphate pH 7, and frozen at - 80 degrees C in 10 mM phosphate, pH 7 plus 0.4 M NaC1. |
Comments | On aerated medium. See table for details |
Entered by | Uri M |
ID | 104025 |