| Reference |
Bremer, H., Dennis, P. P. (1987) Modulation of chemical composition and other parameters of the cell by growth rate. Neidhardt, et al. eds. Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology, 1st ed. chapter 96, Table 2 pp.1530-1 |
| Comments |
Note-following quote is from 2nd edition of book, chapter 97, but pertains to same table:"Table 2 lists the amounts of protein, RNA, and DNA and related physiological parameters for cultures
of E. coli B/r growing exponentially at 37°C in different growth media at rates between 0.6 and 2.5
doublings per h. The per-mass values (top section) represent averages obtained from curves drawn as a best
fit through individually measured points (28). The actual measurements fluctuate by about 15% around
these curves. Most of this scatter represents a true variation from culture to culture (the contribution due to
measuring errors is about ±6%, 2.5%, 5%, and 5% for protein, RNA, DNA, and cell number, respectively).
Protein, RNA, and DNA were measured colorimetrically, and cell numbers were determined with an
electronic particle counter as indicated in Table 2. From the per-mass values, protein and RNA per genome
and protein, RNA, and DNA per cell were calculated." Table gives Protein/mass, RNA/mass, DNA/mass, cell no./mass, protein/genome, RNA/genome, origins/genome, protein/origin and more parameters at 5 different doubling times. See notes beneath table |