DNA synthesis rate in exponentially growing E. coli Br as a function of growth rate at 37 degrees celsius

Range 585-933 nucleotides/sec
Organism Bacteria Escherichia coli
Reference Bremer, H., Dennis, P. P. (1996) Modulation of chemical composition and other parameters of the cell by growth rate. Neidhardt, et al. eds. Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology, 2nd ed. chapter 97 Table 3 Table - link
Method Note 'v' beneath table: "The C period (time required to replicate the chromosome) was determined from age fractionated cultures (Helmstetter et al., 1968 PMID 4866336), synchronized cultures (Bremer et al. 1981 PMID 7022029), flow cytometry data (Skarstad et al., 1986 PMID 3527695), and perhaps most accurately in nonsychronous exponential cultures by measuring the increase in the amount of DNA in the culture after treatment with rifampin and chloramphenicol (Churchward et al., 1977 PMID 324977). The values obtained by these different methods agree to within 10%. The values are considered to be the best average of the reported data."
Comments 585 nts/sec for doubling time of 100 min, 933 nts/sec for doubling time of 24min. See BNID 104928. Multiplication of these values by the corresponding C period (time to replicate chromosome one row under in table), gives ~2.35Mb which is ~half the E. coli genome of 4.6Mb, BNID 100 269 and the number of nucleotides polymerized by each of the two replication forks. See BNID 103995
Entered by Uri M
ID 104938