| Value |
100
copies per chromosome
|
| Organism |
Bacteria Bacillus cereus |
| Reference |
Turgeon N, Laflamme C, Ho J, Duchaine C. Evaluation of the plasmid copy number in B. cereus spores, during germination, bacterial growth and sporulation using real-time PCR. Plasmid. 2008 Sep60(2):118-24.PubMed ID18582938
|
| Method |
Plasmids were introduced into B. cereus ATCC14579 strain using the previously described electroporation protocol (Turgeon et al., 2006). All Bacillus strains were grown at 30 °C, 225 rpm in trypticase soy broth (TSB) (Difco Laboratories, Detroit, MI) or on trypticase soy agar (TSA) (Difco Laboratories). PCN normalized with the 16S gene copy number. |
| Comments |
The plasmid pC194 utilizes rolling circle replication mechanism of group III according to the Osborn classification (Osborn, 1999 Osborn, M., 1999. Database of plasmid replicons (DPR). [On line]. link) |
| Entered by |
Uri M |
| ID |
103866 |