copies per chromosome
||Bacteria Bacillus cereus
||Turgeon N, Laflamme C, Ho J, Duchaine C. Evaluation of the plasmid copy number in B. cereus spores, during germination, bacterial growth and sporulation using real-time PCR. Plasmid. 2008 Sep60(2):118-24.PubMed ID18582938
||Plasmids were introduced into B. cereus ATCC14579 strain using the previously described electroporation protocol (Turgeon et al., 2006). All Bacillus strains were grown at 30 °C, 225 rpm in trypticase soy broth (TSB) (Difco Laboratories, Detroit, MI) or on trypticase soy agar (TSA) (Difco Laboratories). PCN normalized with the 16S gene copy number.
||pLS1 utilizes rolling circle replication mechanism of group II