Plasmid copy number of pLS1 plasmid

Value 2 copies per chromosome
Organism Bacteria Bacillus cereus
Reference Turgeon N, Laflamme C, Ho J, Duchaine C. Evaluation of the plasmid copy number in B. cereus spores, during germination, bacterial growth and sporulation using real-time PCR. Plasmid. 2008 Sep60(2):118-24.PubMed ID18582938
Method Plasmids were introduced into B. cereus ATCC14579 strain using the previously described electroporation protocol (Turgeon et al., 2006). All Bacillus strains were grown at 30 °C, 225 rpm in trypticase soy broth (TSB) (Difco Laboratories, Detroit, MI) or on trypticase soy agar (TSA) (Difco Laboratories). PCN normalized with the 16S gene copy number.
Comments pLS1 utilizes rolling circle replication mechanism of group II
Entered by Uri M
ID 103864