| Range |
Table - link
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Cunningham DS, Liu Z, Domagalski N, Koepsel R, Ataai MM, Domach MM. Pyruvate Kinase-Deficient Escherichia coli Exhibits Increased Plasmid Copy Number and Cyclic AMP. J Bacteriol. 2009 May191(9):3041-9 p.3044 table 1PubMed ID19251844
|
| Method |
"Plasmid copy numbers were determined using the agarose gel electrophoresis technique of Pushnova et al. (ref 34, PMID 10933858) with minor modifications. All determinations were made from exponentially growing cells, with an equal OD of cells collected for every measurement. Specifically, the culture volume corresponding to 4 OD units was spun down (25°C, 10,000×g, 3 min), and the medium was discarded." |
| Comments |
"Table 1 shows the growth and copy number results from the temperature shift experiment. After the shift, the growth rate of each strain decreased slightly, and their copy numbers increased as expected. At 37°C, PB25 maintained a fourfold-higher copy number than JM101." See note beneath table |
| Entered by |
Uri M |
| ID |
103860 |