Copy number of plasmids with ColE1 origin of replication

Range 50-70 copies/cell
Organism Bacteria Escherichia coli
Reference Lutz R, Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15 25(6):1203-10 p.1205 table 1PubMed ID9092630
Method "The intracellular copy numbers were determined by comparing luciferase activity of cells harbouring the respective plasmids with the activity in cells containing only a single luciferase expression unit integrated in the chromosome (data not shown)."
Comments "Module II contains an origin of replication which is protected from outside transcriptional readthrough (ref 14) by two terminators." See figure 2
Entered by Uri M
ID 103857