Copy number of plasmids with psC101 origin of replication

Range 10-12 copies/cell
Organism Bacteria Escherichia coli
Reference Lutz R, Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 1525(6):1203-10PubMed ID9092630
Method The intracellular copy numbers were determined by comparing luciferase activity of cells harbouring the respective plasmids with the activity in cells containing only a single luciferase expression unit integrated in the chromosome (data not shown)
Comments Module II contains an origin of replication which is protected from outside transcriptional readthrough (ref 14 ) by two terminators. See figure 2. These numbers are in good agreement with the numbers Dr. Shay Tal got with single cell experiments: 12.5 ± 40% (unpublished data).
Entered by Uri M
ID 103858