| Range |
20-30 copies/cell
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Lutz R, Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 1525(6):1203-10PubMed ID9092630
|
| Method |
The intracellular copy numbers were determined by comparing luciferase activity of cells harbouring the respective plasmids with the activity in cells containing only a single luciferase expression unit integrated in the chromosome (data not shown). |
| Comments |
Module II contains an origin of replication which is protected from outside transcriptional readthrough (ref 14 ) by two terminators. All promoter sequences which are aligned via the -10 hexamer are flanked by Xho I (upstream) and Eco RI (downstream) cleavage sites. |
| Entered by |
Ron Milo - Admin |
| ID |
100260 |