Kd for binding of Elongation Factor-G-GTP to vacant ribosome
| Value | 0.27 µM |
|---|---|
| Organism | Bacteria Escherichia coli |
| Reference | Yu H, Chan YL, Wool IG. The identification of the determinants of the cyclic, sequential binding of elongation factors tu and g to the ribosome. J Mol Biol. 2009 Feb 27 386(3):802-13.PubMed ID19154738 |
| Method | Preparation of ribosomes, [14C]Phe-tRNA, and N-Ac-[14C]Phe-tRNA, Formation of ribosome tRNA complexes, Chemical footprinting, Treatment of ribosome complexes with sarcin or PAP, Binding of EF-G to ribosome complexes |
| Entered by | Uri M |
| ID | 103826 |