| Value |
10.5
µm
Range: ±2.2 µm
|
| Organism |
Human Homo sapiens |
| Reference |
Zhao L, Kroenke CD, Song J, Piwnica-Worms D, Ackerman JJ, Neil JJ. Intracellular water-specific MR of microbead-adherent cells: the HeLa cell intracellular water exchange lifetime. NMR Biomed. 2008 Feb21(2):159-64 Value from personal communication with author (Jeff Neil).PubMed ID17461436
|
| Method |
P.3 2nd paragraph: "HeLa cells were grown as monolayers on polystyrene microcarrier beads (Nalge Nunc, Rochester, NY, USA) of 160–300µm diameter. Cells were cultured in a 95% O2 and 5% CO2 atmosphere (37°C) for 3 days to reach confluence in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% (v/v) fetal calf serum, 2 mM L-glutamine, 100 units/ml penicillin and 100 μg/ml streptomycin." P.3 4th paragraph: "Light-microscopic images of cells on beads were acquired with a Nikon Diaphot 300 microscope." |
| Comments |
Value above from personal communication with author (Jeff Neil). Researchers measured 24 cells and got a radius of 10.5±2.2 µm (mean ±SD). The cells are a half sphere in culture, so the radius can be used to calculate a volume (BNID 103725). |
| Entered by |
Uri M |
| ID |
103788 |