| Value |
0.17
mM
Range: ±0.025 mM
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Sillero MA, De Diego A, Osorio H, Sillero A. Dinucleoside polyphosphates stimulate the primer independent synthesis of poly(A) catalyzed by yeast poly(A) polymerase. Eur J Biochem. 2002 Nov269(21):5323-9.PubMed ID12392566
|
| Method |
Poly(A) polymerase from yeast was prepared. One unit of enzyme is the amount that incorporates 1 nmol of ATP (as AMP) into an acid insoluble form in 1 min at 37 °C. After incubation at 30 degrees or 37 degrees, the reaction mixtures were analyzed by TLC or HPLC |
| Comments |
Km calculated for S[0.5] (substrate at half of Vmax). The presence of Gp4G or Ap4A changed the kinetic from sigmoidal to hyperbolic, |
| Entered by |
Uri M |
| ID |
103420 |