Hill coefficient of PolyA polymerase

Value 1.6 Unitless
Organism Budding yeast Saccharomyces cerevisiae
Reference Sillero MA, De Diego A, Osorio H, Sillero A. Dinucleoside polyphosphates stimulate the primer independent synthesis of poly(A) catalyzed by yeast poly(A) polymerase. Eur J Biochem. 2002 Nov269(21):5323-9.PubMed ID12392566
Method Poly(A) polymerase from yeast was prepared. One unit of enzyme is the amount that incorporates 1 nmol of ATP (as AMP) into an acid insoluble form in 1 min at 37 °C. After incubation at 30 degrees or 37 degrees, the reaction mixtures were analyzed by TLC or HPLC
Comments As value>1, this is a positively cooperative reaction: Once one ligand molecule is bound to the enzyme, its affinity for other ligand molecules increases.
Entered by Uri M
ID 103417