| Comments |
P.541 note above table 2: "Proton potential of E. coli AN387 (EDTA treated cells) incubated with glycerol plus various electron acceptors or with glucose (fermentative conditions): For each condition the intracellular volume (Vin), the membrane potential (Δѱ) and ΔpH were determined in three or more independent experiments. Vin was measured as the difference from the [3H] H2O and the [14C]taurine space by silicone oil centrifugation. Δѱ was calculated from the accumulation of the [14C]Ph4P+ cation in EDTA-treated cells, ΔpH from the distribution of [14C]benzoate. For the calculation of Δѱ, the cytoplasmic concentrations of Ph4P+ ([Ph4P+in]) were taken (Δѱ=59 mV×lg[Ph4P+in]/[Ph4P+ex]). [Ph4P+in] was obtained by the method of Zaritzky et al. (1981) from the total Ph4P+ (Ph4P+cell, corresponding to cytoplasmic plus membrane-bound Ph4P+) accumulated by the bacteria (for details see text). [Ph4P+cell] was calculated from Ph4P+cell/Vin. dc, dry cells." (-140mV) for growth on (Glycerol + O2) substrate, (-105mV) for fermentative growth on Glucose. See BNID 102120, 104083 |