Change in Gibbs free energy for hydrolysis of ATP to ADP and Pi under different growth conditions in exponential growth range

Range 46.2-47.7 Table - link kJ/mol
Organism Bacteria Escherichia coli
Reference Tran QH, Unden G. Changes in the proton potential and the cellular energetics of Escherichia coli during growth by aerobic and anaerobic respiration or by fermentation. Eur J Biochem. 1998 Jan 15 251(1-2):538-43. p.540 table 1PubMed ID9492330
Method P.540 heading of table 1: "Growth was performed in supplemented M9 medium with glycerol (20 mM for growth under oxic, 40 mM for growth under anoxic conditions) or glucose (20 mM) and the electron acceptors given (50 mM, except for O2). In the logarithmic growth phase, samples were drawn and the bacteria were assayed for ATP and ADP. Pi was taken as 20µmol/g dry cells for growth under oxic and anoxic conditions corresponding to 10 mM (Rao et al., 1993 : Shulman et al., 1979 Willsky and Malamy, 1976)." Calculated from the equation: ΔG (Phos)= ΔG (at standard conditions) + RT ln([ATP]/[ADP][Pi]), where R is the gas constant, T = temperature, and 30.5 kJ/mol was used for ΔG at standard conditions.
Comments Low value 46.2 for growth on (Glycerol + nitrate) substrate, High value 47.7 for growth on (Glycerol + O2) substrate. See BNID 101701
Entered by Uri M
ID 103385