NADH/NAD ratio in cytosol in the steady state of photosynthesis

Value ~1e-3 Unitless
Organism Spinach Spinacia oleracea
Reference Heineke D, Riens B, Grosse H, Hoferichter P, Peter U, Flügge UI, Heldt HW. Redox Transfer across the Inner Chloroplast Envelope Membrane. Plant Physiol. 1991 Apr95(4):p. 1136 left column 2nd paragraphPubMed ID16668101
Method Hydroponic growth. Spinach plants were grown in a climatized chamber with a 9 h light/15 h dark cycle and leaves were stopped in liquid N2 after 8.5 h light or 2 h dark. The frozen tissue was ground and lyophilized. The dry material was homogenized by sonication in a mixture of carbon tetrachloride and heptane, and fractionated by density gradient centrifugation.
Comments On the order of 0.001. According to the equation: NADH/NAD= ((Glu) * (malate) * K, * K2)/ ((2-OG) * (Asp)*(H+) ) K, = 6.61 (GOT) and K2 = 2.78. 10-12 (MDH) From the data of Table IV and assuming a pH of 7.2, cytosolic NADH to NAD ratio in the light is calculated as 0.0005
Entered by Uri M
ID 103334