Burst size of cyanomyovirus in Microcystis aeruginosa

Value 85 Virons/burst Range: 50-120 Table - link Virons/burst
Organism Cyanophage MA-LMM01 (Microcystis aeruginosa)
Reference Brown, C.M., Lawrence, J.E., and Campbell, D.A. (2006) Are phytoplankton population density maxima predictable through analysis of host and viral genomic DNA content? J Mar Biol Assossiation UK 86: 491–498. DOI: link p.492 table 1 & p.495 left column 4th paragraph
Primary Source Yoshida T. et al., Isolation and characterization of a cyanophage infecting the toxic cyanobacterium Microcystis aeruginosa. Appl Environ Microbiol. 2006 Feb72(2):1239-47PubMed ID16461672
Method Primary Source p.1240 right column 2nd paragraph: "[investigators] used a semi-one-step growth procedure as described by Sandaa et al. (ref 37). Exponentially growing cultures of M. aeruginosa (300 ml) were inoculated with the pathogen at a multiplicity of infection (MOI) of 0.72 to 0.90. An M. aeruginosa culture inoculated with an autoclaved pathogen suspension served as the control. After inoculation, an aliquot of the cell suspension was collected from each culture every 2 h for 16 h, and the numbers of host cells and pathogens were determined by optical microscopy and the extinction dilution method (refs 25, 41), respectively. In addition, exponentially growing cultures of M. aeruginosa (300 ml) were inoculated with the virus at MOIs of 0 (autoclaved phage culture), 10^–1, 10^–3, 10^–5, 10^–7, and 10^–9 and incubated, and the changes in host cell numbers were monitored over time using optical microscopy."
Comments Ref p.495 left column 4th paragraph:"Doubling the genome copy number in [investigators’] calculations for Synechococcus WH7803 eliminated the discrepancy. A similar correction was applied to the cyanobacteria Microcystis aeruginosa, which resulted in a predicted burst for phage Ma-LMM01 of 66, within the observed burst range of 50 to 120 (primary source)."
Entered by Uri M
ID 103247