Cyanomyovirus burst size in Synechococcus WH7803

Value 41 Virions/burst Range: 22 - 45 Table - link Virions/burst
Organism Cyanophage S-PM2 (Synechococcus WH7803)
Reference Brown, C.M., Lawrence, J.E., and Campbell, D.A. (2006) Are phytoplankton population density maxima predictable through analysis of host and viral genomic DNA content? J Mar Biol Assossiation UK 86: 491–498. DOI: link p.492 table 1 & p.495 left column 4th paragraph
Primary Source Wilson, W.H., Carr, N.G. & Mann, N.H., 1996. The effect of phosphate status on the kinetics of cyanophage infection of the oceanic cyanobacterium Synechococcus sp.WH7803. Journal of Phycology, 32, 506-516 DOI: 10.1111/j.0022-3646.1996.00506.x
Method p.493 left column 2nd paragraph [general burst size measurement method]:"Burst sizes were derived from either indirect estimates from dilution to- extinction assays of infectivity (Suttle, 1993) or direct counts done with flow cytometry or transmission electron microscopy (TEM). Whenever possible, [investigators] used direct count estimates, since viral burst size estimates based on infectivity can underestimate viral particle numbers (Van Etten, 1983 Cotrell & Suttle, 1995)." p.493 right column 3rd paragraph [Specifically for Synechococcus WH7803/SPM2 phage]:"Burst data: two values reported, for phosphate deplete culture and phosphate replete culture. Burst size determined by dilution-to-extinction and plaque assay for infectious particles (primary source)."
Comments Ref p.495 left column 4th paragraph:"For the cyanophages Cyanomyoviridae S-PM2 and Cyanopodoviridae P60, which both infect Synechococcus WH7803, [investigators] initially predicted burst sizes on the basis of one genome copy per host cell. The predicted burst of 12 for S-PM2 was lower than the 22 to 45 observed in nutrient-replete media (primary source)."
Entered by Uri M
ID 104841