| Range |
40000-60000 bp
|
| Organism |
Mouse Mus musculus |
| Reference |
Garcia HG, Grayson P, Han L, Inamdar M, Kondev J, Nelson PC, Phillips R, Widom J, Wiggins PA. Biological consequences of tightly bent DNA: the other life of a macromolecular celebrity. Biopolymers. 2007 Feb 585(2):115-30.PubMed ID17103419
|
| Primary Source |
Tolhuis B, Palstra RJ, Splinter E, Grosveld F, de Laat W. Looping and interaction between hypersensitive sites in the active beta-globin locus. Mol Cell. 2002 Dec10(6):1453-65.PubMed ID12504019
|
| Method |
The Chromosome Conformation Capture (3C) methodology: formaldehyde cross-linking, Eco RI digestion, intramolecular ligation, and PCR-mediated detection of ligation products after reversal of the cross-links. |
| Comments |
Mode of action is activation.In erythroid cells the hypersensitive sites of the locus control region (LCR), located 40-60 kb away from the active genes, come in close spatial proximity with these genes. The intervening chromatin with inactive globin genes loops out. |
| Entered by |
Uri M |
| ID |
103158 |