| Value |
57
pN
|
| Organism |
Bacteriophage phi 29 |
| Reference |
Smith DE, Tans SJ, Smith SB, Grimes S, Anderson DL, Bustamante C. The bacteriophage straight phi29 portal motor can package DNA against a large internal force.Nature. 2001 Oct 18413(6857):748-52.PubMed ID11607035
|
| Method |
Force-measuring laser tweezers are used to follow packaging activity of a single complex in real time. Stalled, partly prepackaged complexes are attached to a polystyrene microsphere by means of the unpackaged end of the DNA. This microsphere is captured in the optical trap and brought into contact with a second bead that is held by a pipette. This bead is coated with antibodies against the phage, so a stable tether is formed between the two beads |
| Comments |
pN=picoNewton=10^-12Newton. |
| Entered by |
Uri M |
| ID |
103126 |