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Vmax to pack DNA in capsid

Value	103 bp/sec Range: ±12 bp/sec
Organism	Bacteriophage phi 29
Reference	Chemla YR, Aathavan K, Michaelis J, Grimes S, Jardine PJ, Anderson DL, Bustamante C. Mechanism of force generation of a viral DNA packaging motor. Cell. 2005 Sep 9122(5):683-92.PubMed ID16143101
Method	a single stalled packaging complex was attached between a microsphere in an optical trap and a microsphere placed on the end of a micropipette. DNA outside the prohead decreases in length over time as it is reeled into the capsid against a constant external force of approximately 5 pN. ATP concentrations range from 5 to 1000 µM, with ADP and inorganic phosphate (Pi) fixed at 5 µM each (the latter added to ensure a defined free energy of hydrolysis, ?G = ?G0 + kBT ln([ATP]/[ADP][Pi])).
Entered by	Uri M
ID	103170