Fraction of thylakoid proteins with known function involved in photosynthesis

Value 42 %
Organism Thale cress Arabidopsis thaliana
Reference Friso G, Giacomelli L, Ytterberg AJ, Peltier JB, Rudella A, Sun Q, Wijk KJ. In-depth analysis of the thylakoid membrane proteome of Arabidopsis thaliana chloroplasts: new proteins, new functions, and a plastid proteome database. Plant Cell. 2004 Feb16(2):478-99.PubMed ID14729914
Method Sequential extractions with salt, detergent, and organic solvents, followed by multidimensional protein separation steps (reverse-phase HPLC and one- and two-dimensional electrophoresis gels), different enzymatic and nonenzymatic protein cleavage techniques, mass spectrometry, and bioinformatics.
Comments Combining all identified proteins in this study with previous analysis of the thylakoid lumen (Peltier et al., 2002Go), 198 proteins were identified, of which 76 (39%) are integral membrane proteins.
Entered by Uri M
ID 103100