| Value |
42
%
|
| Organism |
Thale cress Arabidopsis thaliana |
| Reference |
Friso G, Giacomelli L, Ytterberg AJ, Peltier JB, Rudella A, Sun Q, Wijk KJ. In-depth analysis of the thylakoid membrane proteome of Arabidopsis thaliana chloroplasts: new proteins, new functions, and a plastid proteome database. Plant Cell. 2004 Feb16(2):478-99.PubMed ID14729914
|
| Method |
Sequential extractions with salt, detergent, and organic solvents, followed by multidimensional protein separation steps (reverse-phase HPLC and one- and two-dimensional electrophoresis gels), different enzymatic and nonenzymatic protein cleavage techniques, mass spectrometry, and bioinformatics. |
| Comments |
Combining all identified proteins in this study with previous analysis of the thylakoid lumen (Peltier et al., 2002Go), 198 proteins were identified, of which 76 (39%) are integral membrane proteins. |
| Entered by |
Uri M |
| ID |
103100 |