| Value |
3.2
unitless
Range: ±0.3 unitless
|
| Organism |
Generic |
| Reference |
Swaminathan R, Hoang CP, Verkman AS. Photobleaching recovery and anisotropy decay of green fluorescent protein GFP-S65T in solution and cells: cytoplasmic viscosity probed by green fluorescent protein translational and rotational diffusion. Biophys J. 1997 Apr72(4):1900-7.PubMed ID9083693
|
| Method |
To determine the relative viscosity of cytoplasm compared to water (same viscosity as PBS), t1/2 for the irreversible recovery process (83 ± 6 ms) in cytoplasm was compared to t1/2 measured for diffusion of purified GFP-S65T in solutions of known viscosity |
| Comments |
GFP mutant S65T was purified from recombinant bacteria for solution studies, and expressed in CHO cell cytoplasm. |
| Entered by |
Uri M |
| ID |
102561 |