| Value |
140
genes
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Schweizer, E. MacKechnie, C. Halverson,HO. The Redundancy of Ribosomal and Transfer RNA Genes in Saccharomyces cerevisiae. J. Mol. Biol. 1969. 40, 261-277. abstract, p.269 bottom paragraph, p.271 table 4 & p.274 2nd paragraphPubMed ID5365012
|
| Method |
Abstract 1st paragraph: "DNA-RNA hybridization studies have been performed to determine the number of 4, 18 and 26 s RNA cistrons present in purified nuclear and mitochondrial DNA of Saccharomyces cerevisiae." |
| Comments |
Abstract 3rd paragraph: "Assuming a genome size of yeast nuclear DNA of 1.25×10^10 daltons, the hybridization data correspond to 140 cistrons for ribosomal RNA and to 320 to 400 cistrons for transfer RNA. With the possible exception of a minor homology between 18 and 26 s ribosomal RNA, the competition experiments indicate that there are separate cistrons for all three classes of RNA." P.269 bottom paragraph: "If one accepts a nuclear genome size in yeast of 1.25x10^10 daltons (Teuro, unpublished results), the extent of hybridization between nuclear DNA and ribosomal RNA is consistent with 140 cistrons for each 18 and 26 s rRNA (Table 4)." P.274 2nd paragraph: "Evidence is presented that S. cerevisiae nuclear DNA contains 140 cistrons for both 18 and 26 s ribosomal RNA and 320 to 400 cistrons for total transfer RNA. No homology was detected between either class of RNA and mitochondrial DNA." For ~150 genes see French et al., 2003 PMID 12588976 p.1559 left column 2nd paragraph |
| Entered by |
Phil Mongiovi |
| ID |
101733 |