||Budding yeast Saccharomyces cerevisiae
||Audra Day, Colette Schneider, and Brandt L. Schneider,chapter 6- Yeast Cell Synchronization, Methods Mol Biol. 2004 241: 55-76. p.73 bullet 17.PubMed ID14970646
|| Short protocols in molecular biology, Fred Ausubel et al., 5th ed. Vol. 2 Wiley, New York. pp. 13-9
||P.73 bullet 17: "Cell density is most accurately determined with a Z2 Coulter Counter Channelyzer. However, a spectrophotometer and OD600 absorbance readings can be substituted. One OD600 is equivalent to approx 3×10^7 cells/mL (primary source). However, OD600 absorbance readings are sensitive to the size of cells. In block-and-release protocols, where cell size increases dramatically, OD600 absorbance readings will increase whereas the cell number does not."
||Best to measure at OD<1 to insure linearity. Value depends on strain and conditions to a factor of several fold.
||Ron Milo - Admin