| Value |
7.7
µm^2/s
Range: ±2.5 µm^2/s
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Elowitz MB, Surette MG, Wolf PE, Stock JB, Leibler S. Protein mobility in the cytoplasm of Escherichia coli. J Bacteriol. 1999 Jan181(1):197-203. abstract, P.199 right column top paragraph, p.200 table 1 & p.201 right column bottom paragraphPubMed ID9864330
|
| Method |
FRAP, Fluorescence Recovery After Photobleaching and photoactivation. |
| Comments |
Abstract: "The apparent diffusion coefficient, Da, of GFP in E. coli DH5alpha was found to be 7.7±2.5 µm^2/s." P.199 right column top paragraph: "The average value of Da for all cells is 7.7 μm^2/s, with a standard deviation (SD) of 2.5 μm^2/s. Other common laboratory strains of E. coli showed similar behaviors (Table 1)." P.201 right column bottom paragraph: "[Investigators] have performed FRAP and photoactivation experiments to measure the apparent diffusion coefficients of GFP and GFP fusion proteins in living E. coli cells. Differences with previous FRAP experiments used in other systems included the use of red GFP photoactivation, requiring 10 times less energy than photobleaching, and the analysis of recovery data throughout the cell rather than only in the irradiated spot. The apparent diffusion coefficient for GFP in bacterial cytoplasm is 7.7 ± 2.5 μm^2/s, about 11 times lower than in water (87 μm^2/s) (refs 25, 26) and significantly lower than in eukaryotic cells (∼27 μm^2/s) (ref 25) and mitochondria (20 to 30 μm^2/s) (ref 19)." See PMID 22942021 p.2973 right column top paragraph: "For example, GFP with a molecular weight MW=27kDa and hydrodynamic radius rp=2.8nm is characterized by cytoplasmic DC (Elowitz et al., 1999) Dcyto=7.7±2.5µm^2/s.". See BNID 105898, 105900. Diffusion coefficient of 8µm^2/s for venus yellow fluorescent protein in E. coli was measured by Elf et al (BNID 105477). For diffusion rate of 40kDa dextran in cytoplasm see BNID 100198 |
| Entered by |
Ron Milo - Admin |
| ID |
100193 |