GFP diffusion rate in cytoplasm of k-12 strain grown in rich medium

Value 5.3 µm^2/s Range: ±1.9 table link µm^2/s
Organism Bacteria Escherichia coli
Reference Konopka MC, Shkel IA, Cayley S, Record MT, Weisshaar JC. Crowding and confinement effects on protein diffusion in vivo. J Bacteriol. 2006 Sep188(17):6115-23. p. 6119 table 1 and p.6120 left column top paragraphPubMed ID16923878
Method FRAP- Fluorescence Recovery After Photobleaching.
Comments P.6119 left column 2nd paragraph: "For cells with a single polar VPS [visible plasmolysis space], the mean diffusion coefficient, <D>, is 1.3 μm^2 s^−1, four times smaller than for those with no VPS (<D> = 5.3 μm^2 s^−1). The two distributions of D overlap only slightly (P < 6 × 10^−7 that the two distributions have the same mean Student t test)." P.6119 right column bottom paragraph: "Plasmolysis spaces and the dispersion of diffusion coefficients: At a Δ of 0.28 osmolal, [investigators] observe two distinct cytoplasmic morphologies (without and with VPSs) and two very different corresponding distributions of D. Formation of a VPS reduces <D> from 5.3 ± 1.9 μm^2 s^−1 to 1.3 ± 1.5 μm^2 s^−1 (± σD) and also increases the relative dispersion σD/<D> by a factor of three (Table 1). This surprising effect was reproducible across different days and occurred for both the B strain and the K-12 strain." After upshift in osmolality of 283±5 mOsmolal. See BNID 100193, 105898
Entered by Uri M
ID 105900