Number of cells in colony in YPD
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|Organism||Budding yeast Saccharomyces cerevisiae|
|Reference||Joseph SB, Hall DW. Spontaneous mutations in diploid Saccharomyces cerevisiae: more beneficial than expected. Genetics. 2004 Dec168(4):1817-25PubMed ID15611159|
|Method||Researchers estimated the average number of generations assuming exponential growth from counts of the number of cells per colony. The number of cells per colony was estimated approximately every seven transfers by choosing a single colony from each of 10 petri dishes. They then suspended the colony in 1 ml of water and estimated cell density using a hemacytometer. (Reichert Bright Line, 0.1 mm depth).|
|Comments||YPD Medium is a blend of peptone, yeast extract, and dextrose in optimal proportions for growing most Saccharomyces cerevisiae strains.|
|Entered by||Ron Milo - Admin|