Number of cells in colony in YPD

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Value 1.4e+6 Cells/colony
Organism Budding yeast Saccharomyces cerevisiae
Reference Joseph SB, Hall DW. Spontaneous mutations in diploid Saccharomyces cerevisiae: more beneficial than expected. Genetics. 2004 Dec168(4):1817-25PubMed ID15611159
Method Researchers estimated the average number of generations assuming exponential growth from counts of the number of cells per colony. The number of cells per colony was estimated approximately every seven transfers by choosing a single colony from each of 10 petri dishes. They then suspended the colony in 1 ml of water and estimated cell density using a hemacytometer. (Reichert Bright Line, 0.1 mm depth).
Comments YPD Medium is a blend of peptone, yeast extract, and dextrose in optimal proportions for growing most Saccharomyces cerevisiae strains.
Entered by Ron Milo - Admin
ID 100266