Maximum density of strains in YPD [yeast extract peptone dextrose] medium

Value 2e+8 Cells/ml
Organism Budding yeast Saccharomyces cerevisiae
Reference F. Sherman, Getting started with yeast, Methods Enzymol. 350, 3-41 (2002). p.15 2nd paragraphPubMed ID12073320
Comments P.15 2nd paragraph: "Growth and Size: "Normal" laboratory haploid strains have a doubling time of approximately 90 min in YPD medium (see below) and approximately 140 min in synthetic media during the exponential phase of growth. However, strains with greatly reduced growth rates in synthetic media are often encountered. Usually strains reach a maximum density of 2×10^8 cells/ml in YPD medium. Titers 10 times this value can be achieved with special conditions, such as pH control, continuous additions of balanced nutrients, filtered-sterilized media, and extreme aeration that can be delivered in fermentors."
Entered by Uri M
ID 108256