Enzymes for which kcat/KM is close to the diffusion-controlled limit
|
Generic |
Table - link |
N/A |
105087 |
Stryer et al, Biochemistry... |
Fraction of oxidoreductases that require a coenzyme
|
Generic |
>80 |
% |
113853 |
Hartl J, Kiefer P, Meyer F... |
Average cumulative free energy change from phosphoglucose isomerase (PGI) to pyruvate kinase (PYK) in glycolysis
|
Unspecified |
-46 |
kJ/mol |
112686 |
Park JO et al., Metabolite... |
Driving force for pyruvate kinase in the mammalian cell line iBMK
|
Mouse Mus musculus |
-3.8 |
kJ/mol |
112687 |
Park JO et al., Metabolite... |
Kinetic parameters of glycolysis and pentose phosphate pathway
|
Bacteria Escherichia coli |
Table - link |
N/A |
105992 |
Chassagnole C, Noiso... |
Estimated fraction of genes that encode enzymes of metabolism
|
Rice Oryza sativa |
25 |
% |
105634 |
Dixon RA, Strack D. ... |
Specific activities of tryptophan synthetase and ornithine transcarbamylase
|
Budding yeast Saccharomyces cerevisiae |
Table - link |
N/A |
109471 |
Weiss RL, Kukora JR,... |
Difference between in vivo & in vitro measurements of kinetic parameters
|
Generic |
≤3 |
orders of magnitude |
111410 |
Bar-Even A. et al., The... |
Median turnover number for the entire data set of enzymes and natural substrates
|
Generic |
~10 |
sec^-1 |
111411 |
Bar-Even A. et al., The... |
Median kcat/KM number for the entire data set of enzymes and natural substrates
|
Generic |
~10^5 |
M^-1×sec^-1 |
111412 |
Bar-Even A. et al., The... |
Median KM for the entire data set of enzymes and natural substrates
|
Generic |
~100 |
µM |
111413 |
Bar-Even A. et al., The... |
Standard Gibbs free energy of glycolytic reactions at various pH and ionic strengths
|
Generic |
Table - link |
kJ/mol |
112696 |
Park JO et al., Metabolite... |
Standard Gibbs free energy of reaction and standard error based on component contribution method
|
Generic |
Table - link |
kJ/mol |
112697 |
Park JO et al., Metabolite... |
Turnover rate of Malonyl-coenzyme A reductase
|
Bacteria Escherichia coli |
28 |
1/sec/subunit |
105607 |
Alber B, Olinger M, Rieder A... |
Amount by which absolute glucose uptake rate, normalized to cell volume, is lower in mammalian iBMK cells compared to E. coli or yeast cells
|
Various |
~30 |
fold lower |
112683 |
Park JO et al., Metabolite... |
Net flux through the oxidative pentose phosphate pathway as fraction of glucose uptake
|
Various |
in E. coli 20%: in yeast 3%: in mammalian iBMK cell 2% |
% of glucose uptake |
112684 |
Park JO et al., Metabolite... |
Flux of glucose-derived two-carbon units into the TCA [tricarboxylic acid] cycle consumed the following fraction of pyruvate:
|
Various |
in E. coli 24%: in mammalian iBMK cell 13%: in yeast 2% |
% of pyruvate made |
112685 |
Park JO et al., Metabolite... |
Cellular concentration of glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase
|
Unspecified |
~0.1 |
µM |
111414 |
Bar-Even A. et al., The... |
kcat of reactions associated with:
|
Unspecified |
Central-CE (carbohydrate energy) metabolism 79 s^-1: secondary metabolism 2.5s^-1 |
sec^-1 |
111415 |
Bar-Even A. et al., The... |
Median kcat of isomerases & ligases
|
Unspecified |
isomerases 33.5s^-1: ligases 3.7s^-1 |
sec^-1 |
111416 |
Bar-Even A. et al., The... |